RecombinantOSM, Rat

CAT: 0384-BK0149-01Size: 10 µgDry Ice: NoHazardous: No
CAT#:0384-BK0149-01Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Oncostatin M (OSM) is a multifunctional cytokine, and belongs to Interleukin-6 (IL-6) subfamily, which also includes IL-11, leukemia inhibitory factor (LIF), ciliary neurotropic factor, cardiotrophin-1, and novel neurotropin-1. In vivo, OSM is secreted from activated T cells, monocytes, neutrophils, and endothelial cells. OSM is related to LIF, and shares a receptor with LIF in human. Human OSM can bind to gp130 and recruit OSM Receptor β or LIF Receptor β to form a ternary complex. OSM stimulates the growth of different types of cells, including megakaryocytes, fibroblasts, vascular endothelial cells, and T cells. OSM inhibits the proliferation of several cancer cell lines, such as solid tissue tumor cells, lung cancer cells, melanoma cells, and breast cancer cells. Recombinant Rat Oncostatin M (rrOSM) produced in E.coli is a single non-glycosylated polypeptide chain containing 215 amino acids. A fully biologically active molecule, rrOSM has a molecular mass of 24.5 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
CAS Number
9000-83-3
Endotoxin
< 0.2 EU/μg, determined by LAL method.
Purity
> 95% as analyzed by SDS-PAGE and HPLC.
Bioactivity
ED50 < 10 ng/mL, measured by a cell proliferation assay using Rat Embryo Brain cells, corresponding to a specific activity of > 1x 10ˆ5 units/mg.
Reconstitution
Reconstituted in ddH2O or PBS at 100 μg/ml.
Molecular Weight
24.5 kDa, observed by reducing SDS-PAGE.
Storage Conditions
Lyophilized recombinant Rat Oncostatin M (rrOSM) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rrOSM remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized after extensive dialysis against PBS.
Host or Source
Escherichia coli.
Recommended Usage
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
AA Sequence
MKRGCSSSS PKLLSQLKSQ ANITGNTASL LEPYILHQNL NTLTLRAACT EHPVAFPSED MLRQLSKPDF LSTVHATLGR VWHQLGAFRQ QFPKIQDFPE LERARQNIQG IRNNVYCMAR LLHPPLEIPE PTQADSGTSR PTTTAPGIFQ IKIDSCRFLW GYHRFMGSVG RVFEEWGDGS RRSRRHSPLW AWLKGDHRIR PSRSSQSAML RSLVPR

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