RecombinantFGF-21, Human

• Description :

  Fibroblast Growth Factor-21 (FGF-21) is a metabolic cytokine belonging to the heparin-binding FGF family. Along with FGF-19/15 and FGF-23, FGF-21 is categorized as a member of the atypical FGF subfamily, as it must be complexed to the Klotho co-receptor in order to bind to the FGF receptors and activate the downstream signaling pathway. In vivo FGF-21 is expressed in liver, pancreas, adipose tissue, and skeletal muscle, and it plays a central role in the energy metabolism. The expression of FGF-21 is stimulated by free fatty acids and insulin resistant states and is correlated with whole-body insulin resistance. FGF-21 activates glucose uptake in adipocytes and increases insulin sensitivity, implicating it as a novel target with potential anti-diabetic properties.Recombinant human Fibroblast Growth Factor-21 (rhFGF-21) produced in E.coli is a single non-glycosylated polypeptide chain containing 182 amino acids. A fully biologically active molecule, rhFGF-21 has a molecular mass of 19.5 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

• Endotoxin :

  < 0.2 EU/μg, determined by LAL method.

• Purity :

  > 95% as analyzed by SDS-PAGE and HPLC.

• Bioactivity :

  ED50 < 0.5 µg/mL, measured by a cell proliferation assay using NIH-3T3 cells in the presence of 1.25 µg/mL mouse Klotho and 10 µg/mL heparin, corresponding to a specific activity of > 2x 10ˆ3 units/mg.

• Reconstitution :

  Reconstituted in ddH2O at 100 μg/mL.

• Molecular Weight :

  19.5 kDa, observed by reducing SDS-PAGE.

• Storage Conditions :

  Lyophilized recombinant human Fibroblast Growth Factor-21 (rhFGF-21) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhFGF-21 remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.

• Appearance :

  Sterile Filtered White lyophilized (freeze-dried) powder.

• Formulation :

  Lyophilized after extensive dialysis against PBS.

• Host or Source :

  Escherichia coli.

• Recommended Usage :

  This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

• CAS Number :

  9000-83-3

• AA Sequence :

  GH PIPDSSPLLQ FGGQVRQRYL YTDDAQQTEA HLEIREDGTV GGAADQSPES LLQLKALKPG VIQILGVKTS RFLCQRPDGA LYGSLHFDPE ACSFRELLLE DGYNVYQSEA HGLPLHLPGN KSPHRDPAPR GPARFLPLPG LPPALPEPPG ILAPQPPDVG SSDPLSMVGP SQGRSPSYAS