EIF1 polyclonal antibody

• Background:

  In mammalian cells, translation is controlled at the level of polypeptide chain initiation by initiation factors. Eukaryotic translation initiation factor 1 (eIF1) is crucial for the scanning process in vitro. During the scanning process, eIF1 is a component of a complex involved in recognition of the initiator codon. Translation is also initiated by the role of eIF1 in regulating the activity of ribosomal subunits 43S, 48S and 40S. eIF1 enables 43S ribosomal complexes to discern between cognate and near-cognate initiation codons, sensing the nucleotide content of initiation codons. It is also a promotor, along with eukar-yotic translation initiation factor 1A (eIF1A), for assembly of 48S ribosomal complexes at the initiation codon of a conventional capped mRNA. In addition, eIF1 and eIF1A, together with eukaryotic translation initiation factor 5 (eIF5), function in the formation of stable 40S ribosomal preinitiation complexes.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Eukaryotic translation initiation factor 1; eIF1; A121; Protein translation factor SUI1 homolog Sui1iso1; EIF1; SUI1

• Swiss Prot:

  P41567

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthetic peptide corresponding to residues in Human EIF1.

• Conjugation:

  Unconjugated

• Applications:

  WB

• Dilution:

  WB: 1:500~1:1000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 13 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  EIF1 polyclonal antibody detects endogenous levels of EIF1 protein.

• Applications Notes:

  Western blot (WB) analysis of EIF1 pAb at 1:500 dilution Lane1:A549 whole cell lysate (40ug) Lane2:Raw264.7 whole cell lysate (40ug) Lane3:U-87MG whole cell lysate (40ug) Lane4:THP-1 whole cell lysate (40ug) Lane5:PC3 whole cell lysate (40ug) Lane6:The lung tissue lysate of Rat (40ug) Lane7:The spleen tissue lysate of Mouse (40ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4