EIF3ε (V116) polyclonal antibody

• Background:

  Translation initiation in eukaryotes necessitates the assembly of an 80S ribosomal complex containing methionyl initiator tRNA (Met-tRNAiMet), which is base paired at the initiation codon (AUG, GUG) in eligible transcripts. Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that leads to 80S ribosomal assembly and initiation of translation. Eukaryotic initiation factor 3 (eIF3) is the largest family of eIFs and consists of at least 12 unique subunits in mammals. eIFε, also known as eIF p47, binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA and associates with the complex p170-eIF3.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  EIF3S5; EIF3F; Eukaryotic translation initiation factor 3 subunit F; eIF3f; Deubiquitinating enzyme eIF3f; EC=3.4.19.12; Eukaryotic translation initiation factor 3 subunit 5; eIF-3-epsilon; eIF3 p47

• Swiss Prot:

  O00303

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide, corresponding to amino acids 91-140 of Human eIF3ε.

• Conjugation:

  Unconjugated

• Applications:

  WB IHC

• Dilution:

  WB: 1:500~1:1000 IHC: 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 47 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  EIF3ε (V116) polyclonal antibody detects endogenous levels of eIF3ε protein.

• Applications Notes:

  Western blot (WB) analysis of eIF3ε (V116) pAb at 1:1000 dilution Lane1:The Testis tissue lysate of Mouse (40ug) Lane2:The Testis tissue lysate of Rat (40ug) Lane3:Hela whole cell lysate (40ug) Lane4:Panc1 whole cell lysate (40ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4