EIF5 polyclonal antibody

• Background:

  Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]) .

• Structure Composition:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Product Name Alternative:

  Eukaryotic translation initiation factor 5; eIF-5

• Swiss Prot:

  P55010

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EIF5. The exact sequence is proprietary.

• Conjugation:

  Unconjugated

• Related Pathways:

  Translational Contral Regulation of elF2, Translational Contral Regulation of elF2

• Applications:

  WB, IHC

• Dilution:

  WB (1/500 - 1/1000), IHC (1/50 - 1/200)

• Purity:

  The antibody was purified by immunogen affinity chromatography.

• Modification:

  Unmodification

• Molecular Weight:

  ~ 49 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Recognizes endogenous levels of EIF5 protein.

• Applications Notes:

  Western blot analysis of EIF5 expression in mouse liver (A), mouse kidney (B), rat kidney (C) whole cell lysates.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4