EIF2α (I45) polyclonal antibody

• Background:

  EIF2α, also known as EIF2S1 or EIF2, is a 315 amino acid subunit of the eukaryotic initiation complex that functions to bind tRNA to the 40S ribosomal subunit (in a GTP-dependent manner), thereby initiating translation. In addition, the phosphorylation state of eIF2α controls the rate of tRNA translation. When eIF2α is not phosphorylated, translation occurs at a normal rate. However, upon phosphorylation by one of several kinases, eIF2α is stabilized, thus preventing the GDP/GTP exchange reaction and slowing translation.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha; EIF2A; EIF2S1

• Swiss Prot:

  P05198

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide, corresponding to amino acids 11-60 of Human eIF2α.

• Conjugation:

  Unconjugated

• Related Pathways:

  Translational Contral Regulation of elF2

• Applications:

  WB IHC

• Dilution:

  WB: 1:500~1:1000 IHC: 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 38 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  EIF2α (I45) polyclonal antibody detects endogenous levels of eIF2α protein.

• Applications Notes:

  Western blot (WB) analysis of eIF2α (I45) polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate (40ug) Lane2:H9C2 whole cell lysate (40ug) Lane3:Hela whole cell lysate (40ug) Lane4:NIH-3T3 whole cell lysate (40ug) Lane5:PC12 whole cell lysate (40ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4