PPARβ polyclonal antibody

• Background:

  Peroxisome proliferator-activated receptor-δ (PPARδ, also known as PPARβ or PPARβ/δ) is a widely expressed member of the PPAR nuclear receptor family, which controls lipid homeostasis . In response to various ligands, PPAR proteins heterodimerize with retinoid X receptors (RXRs) in order to bind DNA and regulate target genes. PPARδ plays a role in many different biological functions, including cholesterol efflux, embryo implantation, preadipocyte proliferation, and wound healing (5-8) . PPARδ has been implicated in colorectal cancer (CRC), as it is normally downregulated by APC, a tumor suppressor frequently knocked out in CRCs . More recently, high fat diets have been found to induce PPARδ in intestinal stem cells and progenitors, increasing their tumorigenicity. Furthermore, in Huntington's disease (HD) mouse models, it was shown that PPARδ was unable bind to huntingtin protein when mutated, which repressed its function. Agonist-induced activation of PPARδ in HD model mice improved cognitive function and increased survival time.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.

• Product Name Alternative:

  Peroxisome proliferator-activated receptor delta; PPAR-delta; NUCI; Nuclear hormone receptor 1; NUC1; Nuclear receptor subfamily 1 group C member 2; Peroxisome proliferator-activated receptor beta; PPAR-beta; PPARD; NR1C2; PPARB

• Swiss Prot:

  Q03181

• Reactivity:

  Human

• Immunogen:

  Synthetic peptide, corresponding to Human PPARβ.

• Conjugation:

  Unconjugated

• Applications:

  WB IHC

• Dilution:

  WB: 1:1000~1:2000 IHC: 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 51 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  PPARβ polyclonal antibody detects endogenous levels of PPARβ protein.

• Applications Notes:

  Western blot (WB) analysis of PPARβ polyclonal antibody at 1:1000 dilution Lane1:EC9706 whole cell lysate (40ug) Lane2:U-87MG whole cell lysate (40ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4