PPAR-α (G17) polyclonal antibody

• Background:

  Peroxisome proliferators are nongenotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family, termed Peroxisome Proliferator Activated Receptors (PPARs) . Nuclear hormone receptors are ligand dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 A6 (CYP450 A6) through interaction with specific response elements. PPAR alpha is activated by free fatty acids including linoleic, arachidonic, and oleic acids. Induction of peroxisomes by this mechanism leads to a reduction in blood triglyceride levels. PPAR alpha is expressed mainly in skeletal muscle, heart, liver, and kidney and is thought to regulate many genes involved in the beta-oxidation of fatty acids. Activation of rat liver PPAR alpha has been shown to suppress hepatocyte apoptosis. PPAR alpha, like several other nuclear hormone receptors, heterodimerizes with retinoic X receptor (RXR) alpha to form a transcriptionally competent complex.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  PPARA; PPAR-alpha; Nuclear receptor subfamily 1 group C member 1; PPARA; NR1C1; PPAR

• Swiss Prot:

  Q07869

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide, corresponding to Human PPAR-α.

• Conjugation:

  Unconjugated

• Applications:

  WB IF

• Dilution:

  WB: 1:500~1000 IF: 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 52 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  PPAR-α (G17) polyclonal antibody detects endogenous levels of PPAR-α protein.

• Applications Notes:

  Western blot (WB) analysis of PPAR-α (G17) pAb at 1:500 dilution Lane1:HepG2 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:THP-1 whole cell lysate (40ug) Lane4:AML-12 whole cell lysate (40ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4