PPAR-α Polyclonal Antibody

• Background:

  Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. PPARA (peroxisome proliferator activated receptor alpha) encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for PPARA, although the full-length nature of only two has been determined.

• Description:

  Rabbit Anti-PPAR-α Polyclonal Antibody

• Product Name Alternative:

  PPARA; NR1C1; PPAR; Peroxisome proliferator-activated receptor alpha; PPAR-alpha; Nuclear receptor subfamily 1 group C member 1

• Gene ID:

  5465

• Accession Number:

  Q07869

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthesized peptide derived from human PPAR-α around the non-phosphorylation site of S21

• Clonality:

  Polyclonal

• Tag:

  Peroxisome proliferator-activated receptor alpha

• Applications:

  WB, IHC, IF, ELISA

• Purification Method:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration:

  1 mg/ml

• Molecular Weight:

  52kD

• Precautions:

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• Shipping Conditions:

  Gel pack with blue ice.

• Storage Conditions:

  Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

• Notes:

  PPAR-α Polyclonal Antibody detects endogenous levels of PPAR-α protein.

• Observed Molecular Weight:

  52

• Formulation:

  Liquid solution

• Buffer pH:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Applications Notes:

  Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; ELISA 1:10000-20000; IHC 1:50-300; IF 1:50-200

• Other Product Names:

  PPARA; NR1C1; PPAR; Peroxisome proliferator-activated receptor alpha; PPAR-alpha; Nuclear receptor subfamily 1 group C member 1

• Tested Applications:

  WB, IHC, IF, IP, ELISA

• Host or Source:

  Rabbit

• Accession Number URL:

  http://www.uniprot.org/uniprot/Q07869

• CAS Number:

  9007-83-4

• Isotype:

  Rabbit IgG