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PPARγ (S112) polyclonal antibody

CAT: 0384-AP0606-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-AP0606-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Peroxisome proliferator-activated receptor γ (PPARγ) is a member of the ligand-activated nuclear receptor superfamily and functions as a transcriptional activator. PPARγ is preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage. Besides its role in mediating adipogenesis and lipid metabolism, PPARγ also modulates insulin sensitivity, cell proliferation and inflammation. PPARγ transcriptional activity is inhibited by MAP kinase phosphorylation of PPARγ at Ser84.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Product Name Alternative
Peroxisome proliferator-activated receptor gamma; Short name=PPAR-gamma; Nuclear receptor subfamily 1 group C member 3; PPARG; NR1C3; PPAR γ; PPARγ
Swiss Prot
P37231
Reactivity
Human, Rat, Mouse
Immunogen
Synthetic peptide, corresponding to Human PPARγ.
Conjugation
Unconjugated
Related Pathways
MAPK Erk in Growth and Differentiation Pathway (, MTOR signaling
Applications
WB IF
Dilution
WB: 1:500~1:1000 IF: 1:100~1:500
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 65 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
PPARγ (S112) polyclonal antibody detects endogenous levels of PPARγ protein.
Applications Notes
Western blot (WB) analysis of PPARγ (S112) polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate (30ug) Lane2:CT-26 whole cell lysate (30ug) Lane3:The Stomach tissue lysate of Mouse (30ug) Lane4:The Heart tissue lysate of Rat (30ug)
Host or Source
Rabbit

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