PPARγ (Phospho-Ser112) polyclonal antibody

• Background:

  Peroxisome proliferator-activated receptor γ (PPARγ) is a member of the ligand-activated nuclear receptor superfamily and functions as a transcriptional activator. PPARγ is preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage. Besides its role in mediating adipogenesis and lipid metabolism, PPARγ also modulates insulin sensitivity, cell proliferation and inflammation. PPARγ transcriptional activity is inhibited by MAP kinase phosphorylation of PPARγ at Ser84.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

• Product Name Alternative:

  Peroxisome proliferator-activated receptor gamma; Short name=PPAR-gamma; Nuclear receptor subfamily 1 group C member 3; PPARG; NR1C3; PPAR γ; PPARγ

• Swiss Prot:

  P37231

• Reactivity:

  Human, Rat, Mouse

• Immunogen:

  Synthetic phosphopeptide derived from human PPARγ around the phosphorylation site of Serine 112.

• Conjugation:

  Unconjugated

• Related Pathways:

  MAPK Erk in Growth and Differentiation Pathway (, MTOR signaling

• Applications:

  WB IP

• Dilution:

  WB: 1:500~1:1000 IP: 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Phosphorylated

• Molecular Weight:

  ~ 67 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  PPARγ (Phospho-Ser112) polyclonal antibody detects endogenous levels of PPARγ protein only when phosphorylated at Ser112.

• Applications Notes:

  Western blot (WB) analysis of PPARγ (Phospho-Ser112) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate (30ug) Lane2:MCF-7 treated with λ-phosphatase whole cell lysate (30ug) Lane3:PC3 whole cell lysate (30ug) Lane4:PC3 treated with λ-phosphatase whole cell lysate (30ug) Lane5:CT-26 whole cell lysate (30ug) Lane6:PMVEC whole cell lysate (30ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4