Mouse anti Human Sphingosine 1 Phosphate 4 Receptor (CT) (EDG6), conjugated with FITC

• Background:

  EDG6 (S1P4) belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. The ligand for EDG6 (S1P4) is sphingosine-1-phosphate. There are 8 known members of the EDG receptor family and they are implicated in mediating growth related effects such as induction of cellular proliferation, alterations in differentiation and survival and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C and several protein tyrosine kinases. EDG6 (S1P4) is expressed in lymphoid and hematopoietic tissue and in the lungs. _x000B_Recently, the designation of the EDG receptors has been modified to include the ligand for the receptor. Thus, the new designation for the EDG6 (S1P4) receptor is the sphingosine 1-phosphate receptor-4 (S1P4).

• Synonyms:

  Endothelial Cell differentiation gene 6 C-terminal, Sphingosine-1-Phosphate Receptor-4 (S1P4)

• UniProt:

  O95977

• Host:

  Mouse

• Species Reactivity:

  Human

• Isotype:

  IgG1

• Clone:

  1

• Conjugation:

  FITC Conjugated

• Type:

  Monoclonal Antibody

• Source:

  Hybridoma produced by the fusion of splenocytes from mice immunized with a unique synthetic peptide derived from the C-terminal region of the EDG6 (S1P4) protein and mouse myeloma cells.

• Applications:

  WB, Flow Cytometry

• Field of Research:

  Signal Transduction

• Purification Method:

  Protein A/G Chromatography

• Assay Principle:

  The X1626M antibody can be used for flow cytometry (use 1 µg of antibody per 106 cells). NOTE: Epitope for the antibody is the C-terminal region of the EDG6 protein. The C-terminus is an intracellular region and cells need to be permeablized prior to staining with antibody. Optimal concentration should be evaluated by serial dilutions.

• Stability:

  See expiration date on vial

• Concentration:

  See vial for Concentration

• Form:

  Provided as solution in phosphate buffered saline with 0,08% sodium azide

• Precautions:

  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

• References & Citations:

  1. Yamazaki, Y., et al. 'EDG-6 as a putative sphingosine 1-phosphate receptor coupling to Ca(2+) signaling pathway.' Biochem. Biophys. Res. Commun. 2000, 268, 583-589._x000D_ 2. Van Brocklyn, J.R., et al. 'Sphingosine 1-phosphate is a ligand for the G protein-coupled receptor EDG-6.' Blood 2000, 95, 2624-2629._x000D_ 3. Takuwa, Y., et al. 'Subtype-specific, differential activities of the EDG family receptors for sphingosine 1-phosphate, a novel lysophospholipid mediator.' Mol. Cell Endocrinol. 2001, 177, 3-11._x000D_ 4. Ohotski, J., et al. Expression of sphingosine 1-phosphate receptor 4 and sphingosine kinase 1 is associated with outcome in oestrogen receptor-negative breast cancer. Br. J. Can. (2012), 106, 1453-1459

• Shipping Conditions:

  Ambient Temperature

• Storage Conditions:

  Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles

• Functional Analysis:

  Flow Cytometry Staining

• CAS Number:

  9007-83-4