NKCC2 Antibody

• Background:

  NKCC2 (Na⁺-K⁺-2Cl⁻ cotransporter 2), encoded by SLC12A1, is a renal-specific transporter located in the thick ascending limb of the loop of Henle. Although classically associated with renal sodium reabsorption and targeted by loop diuretics, NKCC2’s role in the brain is gaining attention. Emerging studies suggest that NKCC2 may influence cerebrospinal fluid composition, neuronal excitability, and osmoregulation. Dysregulation of NKCC2 could contribute to altered ion gradients and excitotoxicity, processes implicated in neurodegenerative diseases such as Alzheimer’s and epilepsy. Understanding NKCC2’s extrarenal functions may uncover novel mechanisms of ion homeostasis and therapeutic opportunities in neurodegeneration.

• Description:

  Rabbit Anti-Rat NKCC2 Polyclonal

• Specifications:

  Detects ~160kDa.

• Product Name Alternative:

  SLC12A1, sodium potassium chloride cotransporter 2, sodium-potassium-chloride cotransporter isoform 2, BSC1, MBSC1, Na-K-2Cl cotransporter, NKCC2A variant A, RBSC

• UNSPSC:

  12352203

• UN Code:

  Non-hazardous

• Hazard Statement:

  Non-hazardous

• Gene ID:

  25065

• Swiss Prot:

  Q5PQV1

• Accession Number:

  NP_001257546.1

• Cellular Locus:

  Membrane

• Host:

  Rabbit

• Species Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Produced against a synthetic peptide mapping to a segment (amino acids 33-55) of the N-terminal tail of rat NKCC2 (antibody designation L224) .

• Target:

  NKCC2

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Polyclonal

• Applications:

  WB | IHC

• Validated Applications:

  WB, IHC

• Field of Research:

  Cancer | Neuroscience | Pumps/Transporters | Ion Pumps/Transporters

• Purification:

  Protein A Purified

• Detection Range:

  1 µg/ml of SPC-401 was sufficient for detection of NKCC2 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

• Concentration:

  1 mg/ml

• Dilution:

  WB (1:1000) ; IHC (1:25) ; optimal dilutions for assays should be determined by the user.

• Weight:

  0.012

• Buffer:

  PBS, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated

• Precautions:

  Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• References & Citations:

  1. Caceres P.S., Ares G.R., and Ortiz P.A. (2009) J Biol Chem. 284 (37) : 24965-24971. 2. Xue H., et al. (2009) Neurogastroentrol Motil. 21 (10) : 1068-1089.

• Shipping Conditions:

  Blue Ice or 4ºC

• Storage Conditions:

  -20ºC

• Specificity:

  Detects ~160kDa.

• Species:

  Human | Mouse | Rat

• CAS Number:

  9007-83-4

• Location:

  Membrane

• Immunogen Species:

  Rat