NKCC2 Antibody (Biotin)

CAT: 0013-GTR18029426Size: 100 µgDry Ice: NoHazardous: No

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CAT#:0013-GTR18029426Size:100 µg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

Rabbit polyclonal to NKCC2 (Biotin) . NKCC2, a kidney-specific member of the cation chloride co-transporter family SLC12, facilitates transport of sodium, potassium, and chloride ions from the lumen of the renal thick ascending limb into the cell. Transport of sodium dilutes the luminal fluid, decreasing its osmolality creating an osmotic driving force for water reabsorption in the connecting tubule and cortical collecting duct under the influence of the hormone vasopressin. NKCC2 is found on the apical surface of the thick ascending limb of the loop of Henle and is blocked by loop diuretics such as furosemide..

Product Name Alternative

SLC12A1, sodium potassium chloride cotransporter 2, sodium-potassium-chloride cotransporter isoform 2, BSC1, MBSC1, Na-K-2Cl cotransporter, NKCC2A variant A, RBSC

UniProt

Q5PQV1

Reactivity

Human, Mouse, Rat

Immunogen

Produced against a synthetic peptide mapping to a segment (amino acids 33-55) of the N-terminal tail of rat NKCC2 (antibody designation L224) .

Target

NKCC2

Clonality

Polyclonal

Conjugation

Biotin

Field of Research

Neuroscience

Purification

Protein A Purified

Concentration

1 mg/ml

Dilution

WB (1:1000) ; IHC (1:25)

Molecular Weight

160kDa

Storage Conditions

Conjugated antibodies should be stored according to the product label

Notes

For research use only.

Applications Notes

1 μg/ml was sufficient for detection of NKCC2 in 10 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Tested Applications

IHC, WB

NCBI Accession Number

NP_001257546.1

Host or Source

Rabbit

Preservative

136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate.

Entrez

25065

UniProtKB · Q5PQV1

Protein information

Q5PQV1_RAT · Rattus norvegicus

View on UniProt ↗

Primary accession: Q5PQV1
Review status: UniProtKB unreviewed (TrEMBL)
Gene: Slc12a1
Protein existence: 1: Evidence at protein level
Organism: Rattus norvegicus (Rat)
Taxonomy ID: 10116
Alternative names: —
EC number: —
Processing: —
Secondary accessions: —

Protein keywords

Cellular component

Cell membraneMembrane

Ligand

ChloridePotassiumSodium

Domain

Coiled coilTransmembraneTransmembrane helix

PTM

GlycoproteinPhosphoprotein

Biological process

Ion transportPotassium transportSodium transportSymportTransport

Technical term

Reference proteome

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Alternative Products

Catalog	Name
MBS805479-01	NKCC2 Antibody: Biotin
NBP2-98480B	Rabbit Polyclonal NKCC2/SLC12A1 Antibody [Biotin]
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MBS9611423-01	NKCC2 Antibody
MBS805636-01	NKCC2 Antibody: PerCP
MBS805913-01	NKCC2 Antibody: HRP
MBS806623-01	NKCC2 Antibody: FITC
MBS806664-01	NKCC2 Antibody: RPE
R32222	SLC12A1 Antibody / NKCC2
R31289	NKCC2 Antibody / SLC12A1