Mouse anti Human CD61

• Background:

  CD61 recognizes an Mr 110-kdalton (kDa) protein, also known as gpIIIa, the common beta-subunit (integrin beta 3-chain) of the gpIIb/IIIa complex and the vitronectin receptor (VNR). The gpIIb/IIIa complex and the VNR are integrins, ie, a/b-heterodimeric glycoprotein complexes that are involved in cell adhesion. With the CD41 antigen (gpIIb or aIIb), the CD61 antigen forms the gpIIb/IIIa complex, which acts as a receptor for fibrinogen, von Willebrand factor (vWf), fibronectin and vitronectin on activated platelets. With the CD51 antigen (VNR a-chain or av), the CD61 antigen forms the VNR, which mediates activation-independent cell adhesion to vitronectin, vWf, fibrinogen, and thrombospondin. The CD61 antigen is found on all normal resting and activated platelets. The CD61 antigen is also found on endothelial cells, megakaryocytes, on some myeloid, erythroid and T-lymphoid leukemic cell lines.

• Synonyms:

  CD61

• UniProt:

  P05106

• Host:

  Mouse

• Species Reactivity:

  Human

• Isotype:

  IgG1

• Clone:

  43.PL

• Conjugation:

  Unconjugated

• Type:

  Monoclonal Antibody

• Source:

  Derived from the hybridization of mouse X63.Ag8.653 myeloma cells with spleen cells of BALB/c mice immunized with purified platelet membrane glycoproteins

• Applications:

  Flow Cytometry

• Field of Research:

  Flow Cytometry, Immunology

• Purification Method:

  Protein A/G Chromatography

• Assay Principle:

  PBMC: Add10 µL of MAB/10^6 PBMC in 100 µL PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µL of MAB/100 µL of whole blood. Mix gently and incubate for 15 minutes at room temperature (20ºC). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

• Stability:

  See expiration date on vial

• Concentration:

  See vial for Concentration

• Form:

  Provided as solution in phosphate buffered saline with 0,08% sodium azide

• Precautions:

  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

• References & Citations:

  1. von dem Borne AEGKr, Modderman PW, Admiraal LG, Nieuwenhuis KH. Platelet antibodies, the overall results. In: Knapp W,Dorken B, Gilks WR, et al, eds. Leucocyte Typing IV, White Cell Differentiation Antigens. Oxford: Oxford University Press; 1989:950-966._x000D_ 2. Modderman PW. Cluster report: CD61. In: Knapp W, Dorken B, Gilks WR, et al, eds. Leucocyte Typing IV, White Cell Differentiation Antigens. Oxford: Oxford University Press; 1989:1025. _x000D_ 3. Modderman PW. New clusters: CD29/CDw49, CD47, CD51, CD55, and CD61. In: Knapp W, Dorken B, Gilks WR, et al, eds. Leucocyte Typing IV, White Cell Differentiation Antigens. Oxford: Oxford University Press; 1989:1017-1019. _x000D_ 4. Springer TA. Adhesion receptors of the immune system. Nature. 1990;346:425-433. _x000D_ 5. Parmentier S, Kaplan C, Catimel B, McGregor JL. New families of adhesion molecules play a vital role in platelet functions. Immunol Today. 1990;11:225-227. _x000D_ 6. Fijnheer R, Modderman PW, Veldman H, et al. Detection of platelet activation with monoclonal antibodies and flow cytometry. Transfusion. 1990;30:20-25. _x000D_ 7. von dem Borne AEGKr, Modderman PW. Cluster report: CD41. In: Knapp W, Dorken B, Gilks WR, et al, eds. Leucocyte Typing IV, White Cell Differentiation Antigens. Oxford: Oxford University Press; 1989:997-999. _x000D_ 8. Jennings LK, Ashmun RA, Wang WF, Dockter ME. Analysis of Human platelet glycoproteins IIb-IIIa and Glanzmann's thrombasthenia in whole blood by flow cytometry. Blood. 1986;68:173-179.

• Shipping Conditions:

  Ambient Temperature

• Storage Conditions:

  Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles

• Functional Analysis:

  Flow Cytometry Staining

• CAS Number:

  9007-83-4