Mouse anti Human IgM (class specific), conjugated with TRITC

• Background:

  The reactivity of the antiserum is restricted to a isotype specific determinant of the IgM molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunofluorescence staining of cytoplasmic Ig. To identify the presence of IgM in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect immunofluorescence staining of cytoplasmic IgM and cell membrane IgM. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:100. These data should be interpreted as general recommendations only.

• Host:

  Mouse

• Isotype:

  IgG1

• Clone:

  NI 179

• Conjugation:

  TRITC

• Source:

  Highly purified IgM isolated from Human serum.

• Assay Principle:

  ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.

• Form:

  Purified monoclonal mouse IgG1 kappa conjugated with TRITC, lyophilized from a solution in phosphate buffered saline (pH7,2); Nopreservative added, as it may interfere with the antibody activity; Noforeign protein added

• Precautions:

  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

• Storage Conditions:

  The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C; Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product Diluted solutions should be stored at +4ºC, not refrozen, and preferably used the same day

• CAS Number:

  9007-83-4