CFDA-SE

CAT: 0804-HY-D0938-01Size: 5 mgDry Ice: NoHazardous: No
CAT#:0804-HY-D0938-01Size:5 mg
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Description
CFDA-SE is a fluorescent dye that can penetrate the cell membrane. It can react with the free amine group in the cytoskeleton protein inside the cell, and finally form a protein complex with fluorescence. After entering the cell, CFDA-SE locates in the cell membrane, cytoplasm and nucleus, and the fluorescence staining is strongest in the nucleus[1]. CFDA-SE dye can be uniformly inherited by the cells with cell division and proliferation, and its attenuation is proportional to the number of cell divisions. This phenomenon can be detected and analyzed by flow cytometry under the excitation light of 488 nm, and can be used to detect the proliferation of cells[1].
CAS Number
[150347-59-4]
Product Name Alternative
CFSE; 5 (6) -Carboxyfluorescein diacetate succinimidyl ester; 5 (6) -CFDA N-succinmidyl ester
UNSPSC
12171500
Hazard Statement
H302, H315, H319, H335
Target
Fluorescent Dye
Type
Dye Reagents
Related Pathways
Others
Field of Research
Others
Assay Protocol
https://www.medchemexpress.com/cfda-se.html
Purity
99.01
Solubility
DMSO : 50 mg/mL (ultrasonic)
Smiles
CC(OC1=CC=C(C2(C(C=CC(C(ON3C(CCC3=O)=O)=O)=C4)=C4C(O2)=O)C(C=CC(OC(C)=O)=C5)=C5O6)C6=C1)=O.CC(OC7=CC=C(C8(C(C=C(C(ON9C(CCC9=O)=O)=O)C=C%10)=C%10C(O8)=O)C(C=CC(OC(C)=O)=C%11)=C%11O%12)C%12=C7)=O
Molecular Formula
C29H19NO11
Molecular Weight
557.46
Precautions
H302, H315, H319, H335
References & Citations
[1]A Bruce Lyons, et al. Flow cytometric analysis of cell division by dilution of CFSE and related dyes. Curr Protoc Cytom. 2013; Chapter 9:Unit9.11.|[2]Xiuyun Jiang et al. Long-lived pancreatic ductal adenocarcinoma slice cultures enable precise study of the immune microenvironment. Oncoimmunology. 2017; 6 (7) : e1333210.|[3]Banks HT, et al. Quantifying CFSE Label Decay in Flow Cytometry Data. Appl Math Lett. 2013 May 1;26 (5) :571-577.|[4]Cui J, et al. Prostaglandin E3 attenuates macrophage-associated inflammation and prostate tumour growth by modulating polarization. J Cell Mol Med. 2021 Jun;25 (12) :5586-5601.
Shipping Conditions
Blue Ice
Storage Conditions
-20°C (Powder, sealed storage, away from moisture and light)
Scientific Category
Dye Reagents
Clinical Information
No Development Reported

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