CFDA-SE

• Description:

  CFDA-SE is a fluorescent dye that can penetrate the cell membrane. It can react with the free amine group in the cytoskeleton protein inside the cell, and finally form a protein complex with fluorescence. After entering the cell, CFDA-SE locates in the cell membrane, cytoplasm and nucleus, and the fluorescence staining is strongest in the nucleus[1]. CFDA-SE dye can be uniformly inherited by the cells with cell division and proliferation, and its attenuation is proportional to the number of cell divisions. This phenomenon can be detected and analyzed by flow cytometry under the excitation light of 488 nm, and can be used to detect the proliferation of cells[1].

• Product Name Alternative:

  CFSE; 5 (6) -Carboxyfluorescein diacetate succinimidyl ester; 5 (6) -CFDA N-succinmidyl ester

• UNSPSC:

  12171500

• Hazard Statement:

  H302, H315, H319, H335

• Target:

  Fluorescent Dye

• Type:

  Dye Reagents

• Related Pathways:

  Others

• Field of Research:

  Others

• Assay Protocol:

  https://www.medchemexpress.com/cfda-se.html

• Purity:

  99.01

• Solubility:

  DMSO : 50 mg/mL (ultrasonic)

• Smiles:

  CC(OC1=CC=C(C2(C(C=CC(C(ON3C(CCC3=O)=O)=O)=C4)=C4C(O2)=O)C(C=CC(OC(C)=O)=C5)=C5O6)C6=C1)=O.CC(OC7=CC=C(C8(C(C=C(C(ON9C(CCC9=O)=O)=O)C=C%10)=C%10C(O8)=O)C(C=CC(OC(C)=O)=C%11)=C%11O%12)C%12=C7)=O

• Molecular Formula:

  C29H19NO11

• Molecular Weight:

  557.46

• Precautions:

  H302, H315, H319, H335

• References & Citations:

  [1]A Bruce Lyons, et al. Flow cytometric analysis of cell division by dilution of CFSE and related dyes. Curr Protoc Cytom. 2013; Chapter 9:Unit9.11.|[2]Xiuyun Jiang et al. Long-lived pancreatic ductal adenocarcinoma slice cultures enable precise study of the immune microenvironment. Oncoimmunology. 2017; 6 (7) : e1333210.|[3]Banks HT, et al. Quantifying CFSE Label Decay in Flow Cytometry Data. Appl Math Lett. 2013 May 1;26 (5) :571-577.|[4]Cui J, et al. Prostaglandin E3 attenuates macrophage-associated inflammation and prostate tumour growth by modulating polarization. J Cell Mol Med. 2021 Jun;25 (12) :5586-5601.

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  -20°C (Powder, sealed storage, away from moisture and light)

• Scientific Category:

  Dye Reagents

• Clinical Information:

  No Development Reported

• CAS Number:

  [150347-59-4]