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5-CFDA-AM

CAT: 0804-HY-131131-01Size: 5 mgDry Ice: NoHazardous: No
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CAT#:0804-HY-131131-01Size:5 mg
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Description
5-CFDA-AM is a cell-permeable esterase substrate that can be used as an active probe to measure enzyme activity and cell membrane integrity. 5-CFDA-AM is electroneutral and can enter the cell at a lower concentration than CFDA, where it is hydrolysed by intracellular esterases to produce carboxyfluorescein. Carboxyfluorescein contains an additional negative charge and can be better retained in the cell[1].
CAS Number
124412-00-6
UNSPSC
12171500
Target
Fluorescent Dye
Type
Dye Reagents
Related Pathways
Others
Field of Research
Others
Assay Protocol
https://www.medchemexpress.com/5-cfda-am.html
Purity
99.81
Solubility
DMSO : 125 mg/mL (ultrasonic)
Smiles
O=C(C1=CC2=C(C3(C4=C(OC5=C3C=CC(OC(C)=O)=C5)C=C(OC(C)=O)C=C4)OC2=O)C=C1)OCOC(C)=O
Molecular Formula
C28H20O11
Molecular Weight
532.45
References & Citations
[1]Elena Gorokhova, et al. A comparison of TO-PRO-1 iodide and 5-CFDA-AM staining methods for assessing viability of planktonic algae with epifluorescence microscopy. J Microbiol Methods. 2012 Jun;89 (3) :216-21.
Shipping Conditions
Blue Ice
Storage Conditions
-20°C (Powder, protect from light)
Scientific Category
Dye Reagents
Clinical Information
No Development Reported

Chemical Information

Cfda-AM

is an indirect measure of cell membrane integrity-are noninvasive and can be monitored conveniently directly in multiwell plates

CAS Number124412-00-6
PubChem CID180237
IUPAC Nameacetyloxymethyl 3',6'-diacetyloxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylate
Molecular FormulaC28H20O11
Molecular Weight532.4
XLogP3.5
Topological Polar Surface Area141
Hydrogen Bond Donor Count0
Hydrogen Bond Acceptor Count11
Rotatable Bond Count9
Heavy Atom Count39
Formal Charge0
Complexity967
SMILES
CC(=O)OCOC(=O)C1=CC2=C(C=C1)C3(C4=C(C=C(C=C4)OC(=O)C)OC5=C3C=CC(=C5)OC(=O)C)OC2=O
InChI
InChI=1S/C28H20O11/c1-14(29)34-13-35-26(32)17-4-7-21-20(10-17)27(33)39-28(21)22-8-5-18(36-15(2)30)11-24(22)38-25-12-19(37-16(3)31)6-9-23(25)28/h4-12H,13H2,1-3H3
InChIKey
YDBUJZYYYBVSEF-UHFFFAOYSA-N
Chemical Structure
2D Structure
2D structure of Cfda-AM
CAS: 124412-00-6
CID: 180237
Formula: C28H20O11
MW: 532.4
Interactive 3D Structure
Loading 3D structure...
View on PubChemRaw 3D data
Computed Properties
PropertyValue
Molecular Weight532.4
XLogP33.5
Hydrogen Bond Donor Count0
Hydrogen Bond Acceptor Count11
Rotatable Bond Count9
Exact Mass532.10056145
Monoisotopic Mass532.10056145
Topological Polar Surface Area141 Ų
Heavy Atom Count39
Formal Charge0
Complexity967
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
Covalently-Bonded Unit Count1
Compound Is CanonicalizedYes

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