5-CFDA-AM

• Description :

  5-CFDA-AM is a cell-permeable esterase substrate that can be used as an active probe to measure enzyme activity and cell membrane integrity. 5-CFDA-AM is electroneutral and can enter the cell at a lower concentration than CFDA, where it is hydrolysed by intracellular esterases to produce carboxyfluorescein. Carboxyfluorescein contains an additional negative charge and can be better retained in the cell[1].

• UNSPSC :

  12171500

• Target :

  Fluorescent Dye

• Type :

  Dye Reagents

• Related Pathways :

  Others

• Field of Research :

  Others

• Assay Protocol :

  https://www.medchemexpress.com/5-cfda-am.html

• Purity :

  99.81

• Solubility :

  DMSO : 125 mg/mL (ultrasonic)

• Smiles :

  O=C(C1=CC2=C(C3(C4=C(OC5=C3C=CC(OC(C)=O)=C5)C=C(OC(C)=O)C=C4)OC2=O)C=C1)OCOC(C)=O

• Molecular Formula :

  C28H20O11

• Molecular Weight :

  532.45

• References & Citations :

  [1]Elena Gorokhova, et al. A comparison of TO-PRO-1 iodide and 5-CFDA-AM staining methods for assessing viability of planktonic algae with epifluorescence microscopy. J Microbiol Methods. 2012 Jun;89 (3) :216-21.

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  -20°C (Powder, protect from light)

• Scientific Category :

  Dye Reagents

• Clinical Information :

  No Development Reported

• CAS Number :

  [124412-00-6]