5-CFDA-AM

5-CFDA-AM

• CAS Number: 124412-00-6
• UNSPSC Description: 5-CFDA-AM is a cell-permeable esterase substrate that can be used as an active probe to measure enzyme activity and cell membrane integrity. 5-CFDA-AM is electroneutral and can enter the cell at a lower concentration than CFDA, where it is hydrolysed by intracellular esterases to produce carboxyfluorescein. Carboxyfluorescein contains an additional negative charge and can be better retained in the cell[1].
• Target Antigen: Fluorescent Dye
• Type: Dye Reagents
• Related Pathways: Others
• Field of Research: Others
• Assay Protocol: https://www.medchemexpress.com/5-cfda-am.html
• Purity: 99.81
• Solubility: DMSO : 125 mg/mL (ultrasonic)
• Smiles: O=C(C1=CC2=C(C3(C4=C(OC5=C3C=CC(OC(C)=O)=C5)C=C(OC(C)=O)C=C4)OC2=O)C=C1)OCOC(C)=O
• Molecular Weight: 532.45
• References & Citations: [1]Elena Gorokhova, et al. A comparison of TO-PRO-1 iodide and 5-CFDA-AM staining methods for assessing viability of planktonic algae with epifluorescence microscopy. J Microbiol Methods. 2012 Jun;89(3):216-21.
• Shipping Conditions: Blue Ice
• Storage Conditions: -20°C (Powder, protect from light)
• Clinical Information: No Development Reported