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5-CFDA

CAT: 0804-HY-D0047Size: 50 mgDry Ice: NoHazardous: No
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CAT#:0804-HY-D0047Size:50 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
5-CFDA is a common aliphatic luciferin-line organism. CFDA conducts free diffusion into cells, and then it is hydrolyzed into carboxyl fluorescein (CF) by intracellular non-specific lipase. CF containing portion contains an additional negative charge so that it is better retained in cells, compared to fluorescein dyes[1][2][3].
CAS Number
79955-27-4
Product Name Alternative
5-Carboxyfluorescein diacetate
UNSPSC
12171500
Hazard Statement
H315, H319
Target
Fluorescent Dye
Type
Dye Reagents
Related Pathways
Others
Field of Research
Others
Assay Protocol
https://www.medchemexpress.com/5-CFDA.html
Purity
98.22
Solubility
DMSO : ≥ 100 mg/mL
Smiles
O=C(C1=CC2=C(C3(C4=C(OC5=C3C=CC(OC(C)=O)=C5)C=C(OC(C)=O)C=C4)OC2=O)C=C1)O
Molecular Formula
C25H16O9
Molecular Weight
460.39
Precautions
H315, H319
References & Citations
[1]Yang T, et al. A novel nonradioactive CFDA assay to monitor the cellular immune response in myeloid leukemia. Immunobiology. 2013 Apr;218 (4) :548-53.|[2]Card SD, et al. Assessment of fluorescein-based fluorescent dyes for tracing Neotyphodium endophytes in planta. Mycologia. 2013 Jan-Feb;105 (1) :221-9.|[3]Fang X, et al. Bone marrow-derived endothelial progenitor cells are involved in aneurysm repair in rabbits. J Clin Neurosci. 2012 Sep;19 (9) :1283-6.
Shipping Conditions
Blue Ice
Storage Conditions
-20°C (Powder, protect from light)
Scientific Category
Dye Reagents
Clinical Information
No Development Reported

Chemical Information

5-Carboxyfluorescein Diacetate
CAS Number79955-27-4
PubChem CID133314
IUPAC Name3',6'-diacetyloxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylic acid
Molecular FormulaC25H16O9
Molecular Weight460.4
XLogP3.2
Topological Polar Surface Area125
Hydrogen Bond Donor Count1
Hydrogen Bond Acceptor Count9
Rotatable Bond Count5
Heavy Atom Count34
Formal Charge0
Complexity828
SMILES
CC(=O)OC1=CC2=C(C=C1)C3(C4=C(C=C(C=C4)C(=O)O)C(=O)O3)C5=C(O2)C=C(C=C5)OC(=O)C
InChI
InChI=1S/C25H16O9/c1-12(26)31-15-4-7-19-21(10-15)33-22-11-16(32-13(2)27)5-8-20(22)25(19)18-6-3-14(23(28)29)9-17(18)24(30)34-25/h3-11H,1-2H3,(H,28,29)
InChIKey
WPUZGNPQMIWOHE-UHFFFAOYSA-N
Chemical Structure
2D Structure
2D structure of 5-Carboxyfluorescein Diacetate
CAS: 79955-27-4
CID: 133314
Formula: C25H16O9
MW: 460.4
Interactive 3D Structure
Loading 3D structure...
View on PubChemRaw 3D data
Computed Properties
PropertyValue
Molecular Weight460.4
XLogP33.2
Hydrogen Bond Donor Count1
Hydrogen Bond Acceptor Count9
Rotatable Bond Count5
Exact Mass460.07943208
Monoisotopic Mass460.07943208
Topological Polar Surface Area125 Ų
Heavy Atom Count34
Formal Charge0
Complexity828
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
Covalently-Bonded Unit Count1
Compound Is CanonicalizedYes

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