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Human CFD/Adipsin/Complement Factor D ELISA Kit PicoKine®

CAT: 0519-EK1199Size: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0519-EK1199Size:96 Well With Removable Strips
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Complement factor D (CFD), also called ADIPSIN or FACTOR D, is a protein which in humans is encoded by the CFD gene. The protein encoded by this gene belongs to the trypsin family of peptidases. It is mapped to 19p13.3. The encoded protein is a component of the alternative complement pathway which is best known for its role in humoral suppression of infectious agents. This protein is also a serine protease that is secreted by adipocytes into the bloodstream. Finally, the encoded protein has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology. What's more, Factor D is a serine protease that stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis.
Description
Human CFD/Adipsin ELISA Kit PicoKine® (96 Tests) . Quantitate Human CFD in cell culture supernatants, cell lysates, serum, plasma (heparin , EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Reproducibility
Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 787 751 817 732 771 32.75 4.2% Sample 2 2378 2440 2261 2414 2373 68.44 2.8% Sample 3 9095 10188 9364 8739 9346 534.02 5.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 787 751 817 732 771 32.75 4.2% Sample 1 787 751 817 732 771 32.75 4.2% Sample 2 2378 2440 2261 2414 2373 68.44 2.8% Sample 2 2378 2440 2261 2414 2373 68.44 2.8% Sample 3 9095 10188 9364 8739 9346 534.02 5.7% Sample 3 9095 10188 9364 8739 9346 534.02 5.7%
Synonyms
Complement factor D;3.4.21.46; Adipsin; C3 convertase activator; Properdin factor D; CFD; DF, PFD
Gene Name
CFD
Gene ID
1675
UniProt
P00746
Reactivity
Human
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Immunogen
Expression system for standard: NS0; Immunogen sequence: I26-A253
Capture Antibody
Monoclonal antibody from mouse
Detection Antibody
Polyclonal antibody from goat
Applications
ELISA
Field of Research
Mesenchymal Stem Cells, Stem Cells
Standard
20 ng/Tube
Sample Type
Cell culture supernatants, cell lysates, serum, plasma (heparin , EDTA) and urine.
Detection Range
312 pg/mL - 20,000 pg/mL
Precision
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 787 2378 90804 804 2193 8576 Standard deviation 44.07 149.81 436.56 49.84 144.73 471.68 CV (%) 6.6%%% 6.6%% 4.8% 6.2% 6.6% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 787 2378 90804 804 2193 8576 Standard deviation 44.07 149.81 436.56 49.84 144.73 471.68 CV (%) 6.6%%% 6.6%% 4.8% 6.2% 6.6% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 787 2378 90804 804 2193 8576 Mean (pg/ml) 787 2378 90804 804 2193 8576 Standard deviation 44.07 149.81 436.56 49.84 144.73 471.68 Standard deviation 44.07 149.81 436.56 49.84 144.73 471.68 CV (%) 6.6%%% 6.6%% 4.8% 6.2% 6.6% 5.5% CV (%) 6.6%%% 6.6%% 4.8% 6.2% 6.6% 5.5%
Sensitivity
<10 pg/mL
Function
Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.
Components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
Storage Conditions
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Calculated Molecular Weight
27033 MW
Specificity
Natural and recombinant human CFD
Anticoagulant
Heparin or EDTA
Gene Name Synonym
Complement factor D
Subcellular Location
Secreted.
Sequence Similarities
Belongs to the peptidase S1 family.
Protein Name
Complement factor D
Sample Data
Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.058 0.132 0.21 0.338 0.604 1.027 1.531 2.037 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.058 0.132 0.21 0.338 0.604 1.027 1.531 2.037 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.058 0.132 0.21 0.338 0.604 1.027 1.531 2.037 O.D. 0.058 0.132 0.21 0.338 0.604 1.027 1.531 2.037
Material Required But Not Provided
Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.

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