ELISA Kit for Complement Factor H (CFH)

CAT: 0338-TRV-0017192-01Size: 24 TestsDry Ice: NoHazardous: No
CAT#:0338-TRV-0017192-01Size:24 Tests
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CAS Number
7732-18-5
Product Name Alternative
CF-H; FH; FHL1; ARMD4; ARMS1; CFHL3; HF1; HF2; HUS; H Factor 2; Age-Related Maculopathy Susceptibility 1; Adrenomedullin binding protein
UniProt
G3V9R2
Applications
Enzyme-linked immunosorbent assay for Antigen Detection.
Field of Research
Metabolic pathway; Infection immunity; Immune molecule
Assay Principle
The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP) -conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Complement Factor H (CFH) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Factor H (CFH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
1. Prepare all reagents, samples and standards;
2. Add 50µ L standard or sample to each well.
        And then add 50µ L prepared Detection Reagent A immediately.
        Shake and mix. Incubate 1 hour at 37° C;
3. Aspirate and wash 5 times;
4. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C;
5. Add 50µ L Stop Solution. Read at 450 nm immediately.
Assay Performance Time
2h
Sample Type
Serum, plasma and other biological fluids
Detection Range
12.35-1,000ng/mL
Precision
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Complement Factor H (CFH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Complement Factor H (CFH) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV
Sensitivity
The minimum detectable dose of this kit is typically less than 5.77ng/mL
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Format
48T, 96T, 96Tx5, 96Tx10, 96Tx100
Specificity
This assay has high sensitivity and excellent specificity for detection of Complement Factor H (CFH) . No significant cross-reactivity or interference between Complement Factor H (CFH) and analogues was observed.
Product Name Synonym
Complement Factor H
Method
Competitive Inhibition
Organism Species
Rattus norvegicus (Rat)

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