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Recombinant e.coli pykF protein

CAT: 0112-ATGP1503-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1503-100Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
PyrF (pyruvate kinase) belongs to the pyruvate kinase family. pykF is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. This process also requires a Magnesium ion. This step is the final one in the glycolytic pathway, which produces pyruvate molecules, the final product of aerobic glycolysis. Recombinant E. coli pykF protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Pyruvate kinase I, ECK1672, JW1666
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
53.3 kDa (494aa)
Additionnal Information
PykF, pyruvate kinase I, ECK1672, JW1666, ATGP1503-10 µg, ATGP1503-20 µg, ATGP1503-50 µg, ATGP1503-100 µg, ATGP1503-250 µg, ATGP1503-500 µg, ATGP1503-1 mg, ATGP1503-10, ATGP1503-20, ATGP1503-50, ATGP1503-100, ATGP1503-250, ATGP1503-500, ATGP1503-1
References & Citations
Liapounova, Na, et al. (2006) Eukaryotic cell 5 (12) : 2138-46, ; ; Valentini G., et al. (2000) J. Biol. Chem. 275:18145-18152
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_416191
Uniprot Accession Number
P0AD61
Species
E.coli
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMKKTKI VCTIGPKTES EEMLAKMLDA GMNVMRLNFS HGDYAEHGQR IQNLRNVMSK TGKTAAILLD TKGPEIRTMK LEGGNDVSLK AGQTFTFTTD KSVIGNSEMV AVTYEGFTTD LSVGNTVLVD DGLIGMEVTA IEGNKVICKV LNNGDLGENK GVNLPGVSIA LPALAEKDKQ DLIFGCEQGV DFVAASFIRK RSDVIEIREH LKAHGGENIH IISKIENQEG LNNFDEILEA SDGIMVARGD LGVEIPVEEV IFAQKMMIEK CIRARKVVIT ATQMLDSMIK NPRPTRAEAG DVANAILDGT DAVMLSGESA KGKYPLEAVS IMATICERTD RVMNSRLEFN NDNRKLRITE AVCRGAVETA EKLDAPLIVV ATQGGKSARA VRKYFPDATI LALTTNEKTA HQLVLSKGVV PQLVKEITST DDFYRLGKEL ALQSGLAHKG DVVVMVSGAL VPSGTTNTAS VHVL

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