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Recombinant E.coli glk protein

CAT: 0112-ATGP3233-050Size: 50 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP3233-050Size:50 µg
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Description
Glk belongs to the bacterial glucokinase family. This protein is not highly important in E. coli as glucose is transported into the cell by the PTS system already as glucose 6-phosphate. Recombinant E. coli glk protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Specifications
Specific activity is > 70 units/mg obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will oxidize 1.0 umole of Glucose to D-glucose 6-phosphate per minute in the presence of Beta-NADP at pH 9.0 at 37C.
Product Name Alternative
Glucokinase, ECK2384, JW2385
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Bioactivity
Specific activity is > 70unit/mg obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will oxidize 1.0 umole of Glucose to D-glucose 6-phosphate per minute in the presence of Beta-NADP at pH 9.0 at 37C.
Molecular Weight
37.1 kDa (344aa) confirmed by MALDI-TOF
Additionnal Information
Glk, Glucokinase, ECK2384, JW2385, ATGP3233-10 µg, ATGP3233-20 µg, ATGP3233-50 µg, ATGP3233-100 µg, ATGP3233-250 µg, ATGP3233-500 µg, ATGP3233-1 mg, ATGP3233-10, ATGP3233-20, ATGP3233-50, ATGP3233-100, ATGP3233-250, ATGP3233-500, ATGP3233-1
References & Citations
Kawai S, Mukai T, et al. (2005) J. Biosci. Bioeng. 99 (4) : 320-30.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol
Scientific Category
Metabolism
NCBI Accession Number
NP_416889
Uniprot Accession Number
P0A6V8
Species
E.coli
AA Sequence
MTKYALV GDVGGTNARL ALCDIASGEI SQAKTYSGLD YPSLEAVIRV YLEEHKVEVK DGCIAIACPI TGDWVAMTNH TWAFSIAEMK KNLGFSHLEI INDFTAVSMA IPMLKKEHLI QFGGAEPVEG KPIAVYGAGT GLGVAHLVHV DKRWVSLPGE GGHVDFAPNS EEEAIILEIL RAEIGHVSAE RVLSGPGLVN LYRAIVKADN RLPENLKPKD ITERALADSC TDCRRALSLF CVIMGRFGGN LALNLGTFGG VFIAGGIVPR FLEFFKASGF RAAFEDKGRF KEYVHDIPVY LIVHDNPGLL GSGAHLRQTL GHIL

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