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Recombinant E.coli dsbC protein

CAT: 0112-HSP0602-500Size: 500 µgDry Ice: NoHazardous: No
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CAT#:0112-HSP0602-500Size:500 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Dsb proteins (DsbA, DsbB, DsbC, and DsbD) catalyze formation and isomerization of protein disulfide bonds in the periplasm of Escherichia coli. DsbC is periplasmic enzyme known as a disulfide isomerase and can convert aberrant disulfide bonds to correct ones. DsbC consists of 236 amino acids containing signal peptide (1-20 amino acids) . Recombinant DsbC was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Thiol:disulfide interchange protein, Protein disulfide isomerase DsbC, Protein disulfide isomerase II, xprA
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
23.6 kDa (217aa) confirmed by MALDI-TOF
Additionnal Information
Disulfide-bond isomerase, DsbC, Disulfide-bond isomerase C, dsbC, xprA, Disulfide-bond isomerase (DsbC) E.Coli, Thiol:disulfide interchange protein dsbC, Disulfide-bond isomerase C Thiol:disulfide interchange protein dsbC ., HSP0602-10 µg, HSP0602-20 µg, HSP0602-50 µg, HSP0602-100 µg, HSP0602-250 µg, HSP0602-500 µg, HSP0602-1 mg, HSP0602-10, HSP0602-20, HSP0602-50, HSP0602-100, HSP0602-250, HSP0602-500, HSP0602-1
References & Citations
Peter W., et al. (2002) . EMBO 21, 4774-4784; ; Laura Segatori., et al. (2004) . PNAS 27, 10018-10023
Other References
Garcin EB, et al. Structural and mechanistic insights into unusual thiol disulfide oxidoreductase. (J Biol Chem. 2012) {https://pubmed.ncbi.nlm.nih.gov/22128175/}
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 2mM EDTA
Scientific Category
Heat Shock Proteins
NCBI Accession Number
CAQ33205.1
Uniprot Accession Number
A0A140N4H6
Species
Human
AA Sequence
MDDAAIQQTL AKMGIKSSDI QPAPVAGMKT VLTNSGVLYI TDDGKHIIQG PMYDVSGTAP VNVTNKMLLK QLNALEKEMI VYKAPQEKHV ITVFTDITCG YCHKLHEQMA DYNALGITVR YLAFPRQGLD SDAEKEMKAI WCAKDKNKAF DDVMAGKSVA PASCDVDIAD HYVLGVQLGV SGTPAVVLSN GTLVPGYQPP KEMKEFLDEH QKMTSGK

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