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Recombinant e.coli gldA protein

CAT: 0112-ATGP3130-004Size: 4 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP3130-004Size:4 µg
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Description
GldA catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone) . This protein allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions. In E. coli, an important role of GldA is also likely to regulate the intracellular level of dihydroxyacetone by catalyzing the reverse reaction, i. e. the conversion of dihydroxyacetone into glycerol. gldA possesses a broad substrate specificity, since it is also able to oxidize 1, 2-propanediol and to reduce glycolaldehyde, methylglyoxal and hydroxyacetone into ethylene glycol, lactaldehyde and 1, 2-propanediol, respectively. Recombinant E. coli gldA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Specifications
Specific activity > 14 Units/ml One unit will oxidize 1.0 umole of glycerol to dihydroxyacetone per minute at pH 8.0 at 25C
Product Name Alternative
Glycerol dehydrogenase, ECK3937, JW5556
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
Enzyme Activity, SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Bioactivity
Specific activity: > 14 Units/ml One unit will oxidize 1.0 umole of glycerol to dihydroxyacetone per minute at pH 8.0 at 25C
Molecular Weight
41.1 kDa (390aa) confirmed by MALDI-TOF
Additionnal Information
GldA, Glycerol dehydrogenase, ECK3937, JW5556, ATGP3130-10 µg, ATGP3130-20 µg, ATGP3130-50 µg, ATGP3130-100 µg, ATGP3130-250 µg, ATGP3130-500 µg, ATGP3130-1 mg, ATGP3130-10, ATGP3130-20, ATGP3130-50, ATGP3130-100, ATGP3130-250, ATGP3130-500, ATGP3130-1
References & Citations
Subedi K.P., et al. (2008) FEMS Microbiol. Lett. 279:180-187; ; Gonzalez R., et al. (2008) Metab. Eng. 10:234-245
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) 10% glycerol
Scientific Category
Signal Transduction
NCBI Accession Number
NP_418380
Uniprot Accession Number
P0A9S5
Species
E.coli
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMDRIIQS PGKYIQGADV INRLGEYLKP LAERWLVVGD KFVLGFAQST VEKSFKDAGL VVEIAPFGGE CSQNEIDRLR GIAETAQCGA ILGIGGGKTL DTAKALAHFM GVPVAIAPTI ASTDAPCSAL SVIYTDEGEF DRYLLLPNNP NMVIVDTKIV AGAPARLLAA GIGDALATWF EARACSRSGA TTMAGGKCTQ AALALAELCY NTLLEEGEKA MLAAEQHVVT PALERVIEAN TYLSGVGFES GGLAAAHAVH NGLTAIPDAH HYYHGEKVAF GTLTQLVLEN APVEEIETVA ALSHAVGLPI TLAQLDIKED VPAKMRIVAE AACAEGETIH NMPGGATPDQ VYAALLVADQ YGQRFLQEWE

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