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Recombinant e.coli sodA protein

CAT: 0112-ATGP1037-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1037-100Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Superoxide dismutase, Mn, also known as sodA, is a member of the iron/manganese superoxide dismutase family. SodA destroys radicals which are normally produced within the cells and which are toxic to biological systems. It works by catalyzing the dismutation of the superoxide radical O2- to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase. Recombinant E. coli sodA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Specifications
Specific activity is > 300 units/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7.5 at 25C.
Product Name Alternative
Superoxide dismutase manganese, Superoxide dismutase, manganese, MnSOD
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
Enzyme Activity, SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Bioactivity
Specific activity is > 300unit/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7.5 at 25C.
Molecular Weight
25.2 kDa (226aa) confirmed by MALDI-TOF
Additionnal Information
SodA, Superoxide dismutase manganese, Superoxide dismutase, manganese, MnSOD, ATGP1037-10 µg, ATGP1037-20 µg, ATGP1037-50 µg, ATGP1037-100 µg, ATGP1037-250 µg, ATGP1037-500 µg, ATGP1037-1 mg, ATGP1037-10, ATGP1037-20, ATGP1037-50, ATGP1037-100, ATGP1037-250, ATGP1037-500, ATGP1037-1
References & Citations
Barrister J V., et al. (1987) Crit Rev Biochem. 22:11-180.; ; ujika I., et al. (1991) Acta Histochem Cytochem. 24:489-495.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
Scientific Category
Redox Proteins
NCBI Accession Number
NP_418344
Uniprot Accession Number
P00448
Species
E.coli
AA Sequence
MGSSHHHHHH SSGLVPRGSH MSYTLPSLPY AYDALEPHFD KQTMEIHHTK HHQTYVNNAN AALESLPEFA NLPVEELITK LDQLPADKKT VLRNNAGGHA NHSLFWKGLK KGTTLQGDLK AAIERDFGSV DNFKAEFEKA AASRFGSGWA WLVLKGDKLA VVSTANQDSP LMGEAISGAS GFPIMGLDVW EHAYYLKFQN RRPDYIKEFW NVVNWDEAAA RFAAKK

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