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E.coli sodA Recombinant Protein

CAT: 0519-PROTP00448-500ugSize: 500 µgDry Ice: NoHazardous: No
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CAT#:0519-PROTP00448-500ugSize:500 µg
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Description
E. coli sodA Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-206aa. Application (s) : Enzyme Activity, SDS-PAGE.
Synonyms
Superoxide dismutase manganese, Superoxide dismutase, manganese, MnSOD
Gene Name
SodA
UniProt
P00448
Tag
His-Tag
Source
E. coli
Applications
Enzyme Activity, SDS-PAGE
Field of Research
Redox Proteins
Purification
> 95% by SDS-PAGE
Concentration
1mg/mL (determined by Bradford assay)
Bioactivity
Specific activity is > 300 units/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7.5 at 25°C.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
25.2 kDa (226aa) confirmed by MALDI-TOF
Contents
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

UniProtKB · P00448

Superoxide dismutase [Mn]

SODM_ECOLI · Escherichia coli (strain K12)

View on UniProt ↗
Primary accession
P00448
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
sodA
Protein existence
1: Evidence at protein level
Organism
Escherichia coli (strain K12)
Taxonomy ID
83333
Alternative names
—
EC number
1.15.1.1
Processing
—
Secondary accessions
Q2M8K5
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

Ligand

ManganeseMetal-binding

Molecular function

Oxidoreductase

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