HCM045 Mannitol Salt Agar

Peptone and beef extract powder provides carbon, nitrogen, vitamins and minerals; D- mannitol to fermentable sugars; higher levels of sodium chloride to provide a higher osmotic pressure, suppress most non-staphylococcal microorganisms ; phenolsulfonphthalein as pH indicator; agar is medium coagulant. Typical pathogenic staphylococci (coagulase positive) D- mannitol produce acid fermentation and produce yellow colonies with a yellow halo, typically non-pathogenic Staphylococcus unfermented D- mannitol to form red colonies. Formulation (per liter) : Pancreatic digest of casein 5.0g Pancreatic digest of animal tissue 5.0g Beef Extract 1.0g Sodium Chloride 75.0g Mannitol 10.0g Phenol Red 0.025g Agar 15.0g Final PH 7.4±0.2 How to use: 1.Suspend 111g in 1L of distilled water , stirring heated to boiling to completely dissolve , autoclave at 121°C for 15 minutes. 2.Diluted and treated samples. Quality control: Item The name and number of strain Growth Colony Color 1 Staphylococcus aureus CMCC (B) 26003 Good Golden yellow 2 Staphylococcus epidermidis CMCC (B) 26069 Good Red 3 Escherichia coli CMCC (B) 44102 Inhibition --

Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.