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HCM015 Urea Agar Base

CAT: 0938-HCM015Size: 500 g/bottleDry Ice: NoHazardous: No
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CAT#:0938-HCM015Size:500 g/bottle
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Assay Principle
Peptone provides the carbon and nitrogen; maintain a balanced osmotic sodium chloride; potassium dihydrogen phosphate is buffers; decomposing bacteria urease urea medium, produce large amounts of ammonia, agar as medium coagulant. Formulation (per liter) : Peptone 1g Sodium chloride 5g Glucose 1g Ppotassium dihydrogen phosphate 2g Phenol red 0.012g Agar 12g Final pH7.2 ± 0.2
Assay Protocol
1.Suspend 21g in 1L of distilled or deionized water. Heat with frequent agitation and boil to completely dissolve the powder. Distribute into flasks. Autoclave at 121°C for 15 minutes. cooling to 50-55 °C and adding 40% urea solution. 2.Diluted and treated samples.
Storage Conditions
Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.
Recommended Usage
For differentiating enteric bacteria based on urease activity by adding 40% sterile urea solution.

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