TIMP-1

• Description:

  TIMP1 is an extracellular inhibitor of MMPs (see above) including MMP1, 2, 3, 7, 8, 9, 10, 11, 12, 13, and 16. It belongs to the I35 (TIMP) family of irreversible protease inhibitors that function as key modulators of extracellular matrix degradation during tissue development and remodeling. TIMP1 can also act through an MMP-independent mechanism to promote erythropoiesis by stimulating proliferation and differentiation of erythroid progenitors. Recombinant human TIMP-1 is a 20.6 kDa protein containing 184 amino acid residues.

• Synonyms:

  Tissue inhibitor of metalloproteinases, Fibroblast collagenase inhibitor, Erythroid-potentiating activity

• NCBI Gene ID:

  7076

• UniProt:

  P01033

• Accession Number:

  NP_003245

• Accession Number mRNA:

  NM_003254

• Chromosomal Location:

  Xp11.3-p11.23

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Sequence:

  CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQ ALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLQGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIA

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 95% by SDS-PAGE & HPLC analyses

• Bioactivity:

  TIMP1 activity was measured by its ability to inhibit human MMP-1 induced hydrolysis of a chromogenic peptide substrate at room temperature. Half maximal inhibition was obtained at a TIMP-1 concentration of approximately 0.5 μg/ml, when using an MMP-1 concentration of 1.6 μg/ml.

• Length:

  184

• Form:

  Lyophilized

• Molecular Weight:

  20.6 kDa

• Host or Source:

  E. coli