4-1BBL / CD137L / TNFSF9 Protein (His Tag)

• Description:

  4-1BB ligand is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. It is shown to be a type II surface glycoprotein belonging to the TNF superfamily. Expression of 4-1BBL is restricted to APCs, such as dendritic cells, macrophages, and activated B cells. Members of the TNF-TNF receptor superfamily have been shown to play critical roles in regulating cellular activation, differentiation and apoptosis. Several studies have reported that 4-1BBL/4-1BB interaction provided a co-stimulatory signal to T cells, and increased T cell proliferation and cytokines production. In addition, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases.

• Synonyms:

  4-1BB-L Protein; Cd137l Protein; Cd157l Protein; Ly63l Protein; Tnfsf9 Protein

• Accession Number:

  NP_033430.1

• Reactivity:

  Mouse

• Cross Reactivity:

  Mouse

• Label:

  His-Tag

• Assay Protocol:

  See Datasheet send with the product

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method

• Purity:

  > 97 % as determined by SDS-PAGE

• Bioactivity:

  1.Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse 4-1BB Ligand at 20 μg/ml (100ul/well) can bind human 4-1BB with a linear range of 15.6-500 ng/mL. 2.Loaded Recombinant Mouse 4-1BB/CD137 Protein on proA Biosensor, can bind Recombinant Mouse 4-1BB Ligand/TNFSF9 Protein with an affinity constant of 0.807 nM as determined in BLI assay (Sartorius Octet RED384) .

• Length:

  222

• Form:

  Lyophilized or frozen liquid

• Buffer:

  PBS, pH 7.4

• Reconstitution:

  See Datasheet send with the product

• Molecular Weight:

  45 kDa

• Storage Conditions:

  Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

• Host or Source:

  HEK 293 cells