4-1BBL

• Description:

  4-1BBL, a member of the TNF superfamily, is expressed in B cells, dendritic cells, activated T cells and macrophages. 4-1BBL binds to its receptor 4-1BB, and provides a co-stimulatory signal for T cell activation and expansion. The human 4-1BBL gene codes for a 254 amino acid type II transmembrane containing a 28 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 205 amino acid extracellular domain. The soluble form of 4-1BBL contains the TNF-like portion of the extracellular domain of 4-1BBL. Recombinant human 4-1BBL is a soluble 19.5 kDa protein consisting of 185 amino acid residues.

• Synonyms:

  TNFSF9; CD137L; 4-1BB-L

• NCBI Gene ID:

  8744

• UniProt:

  P41273

• Accession Number:

  NP_003802.1

• Accession Number mRNA:

  NM_003811.3

• Chromosomal Location:

  19p13.3

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Sequence:

  MREGPELSPDDPAGLLDLRQGMFAQLVAQNVLLIDGPLSWYSDPGLAGVSLTGGLSYKEDTKELVVAKAGVYYVFFQLELRRVVAGEGSGSVSLALHLQPLRSAAGAAALALTVDLPPASSEARNSAFGFQGRLLHLSAGQRLGVHLHTEARARHAWQLTQGATVLGLFRVTPEIPAGLPSPRSE

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 97% by SDS-PAGE & HPLC analyses

• Bioactivity:

  Determined by the dose-dependent stimulation of IL-8 production by human PBMC. The expected ED50 for this effect is 5-10 ng/ml. NOTE: Results may vary with different PBMC donors.

• Length:

  185

• Form:

  Lyophilized

• Molecular Weight:

  19.5 kDa

• Host or Source:

  E. coli