TNFSF9 / 4-1BBL / CD137L Protein (human hFc Tag, ECD)

• Description:

  4-1BB ligand is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. It is shown to be a type II surface glycoprotein belonging to the TNF superfamily. Expression of 4-1BBL is restricted to APCs, such as dendritic cells, macrophages, and activated B cells. Members of the TNF-TNF receptor superfamily have been shown to play critical roles in regulating cellular activation, differentiation and apoptosis. Several studies have reported that 4-1BBL/4-1BB interaction provided a co-stimulatory signal to T cells, and increased T cell proliferation and cytokines production. In addition, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases.

• Synonyms:

  4-1BB-L Protein; 4-1BBL Protein; CD137L Protein; TNLG5A Protein

• Accession Number:

  NP_003802.1

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Label:

  HFc-Tag

• Assay Protocol:

  See Datasheet send with the product

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method

• Purity:

  > 90 % as determined by SDS-PAGE

• Bioactivity:

  1. Immobilized human TNFRSF9 at 10μg/mL (100μL/well) can bind human TNFSF9. The EC50 of human TNFSF9 is 7-40ng/mL. 2. Measured by its ability to induce IL-8 secretion by HT1080 transfected with human CD137. The EC50 for this effect is typically 0.3-1.2 μg/mL.

• Length:

  444

• Form:

  Lyophilized or frozen liquid

• Buffer:

  PBS, pH 7.4

• Reconstitution:

  See Datasheet

• Molecular Weight:

  48 kDa

• Storage Conditions:

  Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

• Host or Source:

  HEK 293 cells