Tyrosine Hydroxylase Antibody

• Background:

  Tyrosine Hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons.

• NCBI Gene ID:

  25085

• Swiss Prot:

  P04177

• Accession Number:

  P04177

• Host:

  Sheep

• Reactivity:

  Human, Mouse, Rat, Dog, Bovine

• Immunogen:

  TH polyclonal antibody was raised against native rat tyrosine hydroxylase, purified from pheochromocytoma.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Neuroscience, Signal Transduction

• Purification:

  Affinity Purified

• Concentration:

  Batch dependent

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For long term storage –80˚ C is recommended, but shorter term storage at –20˚ C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

• Calculated Molecular Weight:

  60

• Applications Notes:

  Tyrosine Hydroxylase antibody is specific for the 60k rat TH in lysates of PC-12 Cells. Applications include Immunofluorescence (IF), Immunohistochemistry (IHC) and Western Blot (WB) . The antibody has been characterized in blot immunolabeling and immunocytochemical studies and cross-reacts with many different mammalian and some non-mammalian forms of TH. When internally tested under ideal conditions the working dilutions were 1:1000 for IHC, IF and WB.

• Symbol:

  Th

• NCBI Official Name:

  Tyrosine hydroxylase

• NCBI Organism:

  Rattus norvegicus

• Background Reference 01:

  Xu, Z.-Q., Lew, J. Y., Harada, K., Aman, K., Goldstein, M., Deutch, A., Haycock, J. W., and Hokfelt, T., "Immunohistochemical studies on phosphorylation of tyrosine hydroxylase in central catecholamine neurons using site- and phosphorylation state-specific antibodies, " Neuroscience, 82 (1998) 727-738.

• Background Reference 02:

  Haycock, J.W. "Multiple forms of tyrosine hydroxylase in human neuroblastoma cells: Quantitation with isoform-specific antibodies, " J. Neurochem. 60 (1993) 493-502.

• Background Reference 03:

  Haycock, J.W., "Four forms of tyrosine hydroxylase are present in human adrenal medulla, " J. Neurochem. 56 (1991) 2139 - 2142.

• Background Reference 04:

  Haycock, J.W., "Quantitation of tyrosine hydroxylase protein levels: Spot immunolabeling with an affinity-purified antibody, " Anal. Biochem. 181 (1989) 259 - 266.

• Tested Applications:

  WB, IF, IHC

• Protein ID:

  136577

• Physical Properties:

  Liquid