Tyrosine Hydroxylase (phospho Ser31) Antibody

• Background:

  Tyrosine Hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons. We raised this polyclonal antibody against a peptide representing the sequence around Ser19 in rat TH purified. This antibody is suitable for most immunochemical applications in a variety of mammalian and some non-mammalian species.

• NCBI Gene ID:

  25085

• Swiss Prot:

  P04177

• Accession Number:

  P04177

• Host:

  Rabbit

• Reactivity:

  Mouse, Rat

• Immunogen:

  TH (Ser31) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser31 of rat tyrosine hydroxylase (TH) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Neuroscience, Signal Transduction, Phospho-Specific

• Purification:

  Affinity Purified

• Concentration:

  Batch dependent

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For long term storage –80˚ C is recommended, but shorter term storage at –20˚ C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

• Calculated Molecular Weight:

  60

• Specificity:

  Tyrosine Hydroxylase antibody is specific for the 60k Tyrosine Hydroxylase phosphorylated at Ser31 in PC-12 cells stimulated by Okadaic Acid.

• Applications Notes:

  Applications include Dot Blots (DB), Immunoblotting (IB), Immunofluorescence (IF), Immunohistochemistry (IHC) and Western Blots (WB) . Mouse and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for IF, IHC, DB and WB.

• Symbol:

  Th

• NCBI Official Name:

  Tyrosine hydroxylase

• NCBI Organism:

  Rattus norvegicus

• Background Reference 01:

  Witkovsky, P., Gabriel, R., Haycock, J. W., and Meller, E., "Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina, " J. Chem. Neuroanat., 19 (2000) 105 - 116.

• Background Reference 02:

  Salvatore, M.F., Garcia-Espana, A., Goldstein, M., Deutch, A.Y., and Haycock, J.W., "Stoichiometry of tyrosine hydroxylase phosphorylation in the nigrostriatal and mesolimbic systems in vivo: effects of acute haloperidol and related compounds, " J. Neurochem., 75 (2000) 225 - 232.

• Background Reference 03:

  Xu, Z.-Q., Lew, J. Y., Harada, K., Aman, K., Goldstein, M., Deutch, A., Haycock, J. W., and Hokfelt, T., "Immunohistochemical studies on phosphorylation of tyrosine hydroxylase in central catecholamine neurons using site- and phosphorylation state-specific antibodies, " Neuroscience, 82 (1998) 727-738.

• Tested Applications:

  WB, IF, IHC

• Protein ID:

  136577

• Physical Properties:

  Liquid