Tyrosine Hydroxylase (Ab 31) Antibody

• Background:

  Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the synthesis of the neurotransmitter dopamine and other catecholamines. TH functions as a tetramer, with each subunit composed of a regulatory and catalytic domain, and exists in several different isoforms . This enzyme is required for embryonic development since TH knockout mice die before or at birth . Levels of transcription, translation and posttranslational modification regulate TH activity. The amino-terminal regulatory domain contains three serine residues: Ser9, Ser31 and Ser40. Phosphorylation at Ser40 by PKA positively regulates the catalytic activity of TH . Phosphorylation at Ser31 by CDK5 also increases the catalytic activity of TH through stabilization of TH protein levels.

• NCBI Gene ID:

  7054

• Swiss Prot:

  P07101

• Accession Number:

  NP_954986.2

• Host:

  Rabbit

• Reactivity:

  Mouse, Rat

• Immunogen:

  Tyrosine Hydroxylase (Ab-31) antibody was raised against a peptide sequence around aa.29~33 (V-T-S-P-R) derived from Mouse Tyrosine Hydroxylase.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Neuroscience

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  55 - 60 kDa

• Specificity:

  This antibody detects endogenous levels of total Tyrosine Hydroxylase protein.

• Applications Notes:

  Western Blot: 1:500~1:1000

• Symbol:

  TH

• NCBI Official Name:

  Tyrosine hydroxylase

• NCBI Organism:

  This antibody was raised against a peptide sequence around aa.29~33 (V-T-S-P-R) derived from Mouse Tyrosine Hydroxylase.

• Background Reference 01:

  Kumer, S. C. and Vrana, K. E. (1996) J Neurochem 67, 443-62. Bodeau-Pean, S. et al. (1999) J Biol Chem 274, 3469-75. Kobayashi, K. et al. (1995) J Biol Chem 270, 27235-43. Lew, J. Y. et al. (1999) Mol Pharmacol 55, 202-9. Vie, A. et al. (1999) J Biol Chem 274, 16788-95. Lindgren, N. et al. (2000) J Neurochem 74, 2470-7. Moy, L. Y. and Tsai, L. H. (2004) J Biol Chem 279, 54487-93. Lehmann, I. T. et al. (2006) J Biol Chem 281, 17644-51. Saraf, A. et al. (2007) J Biol Chem 282, 573-80.

• Other Product Names:

  TYH, DYT14, DYT5b, TYH, TH

• Tested Applications:

  WB

• Protein ID:

  88900501

• Physical Properties:

  Liquid