TOB1 (phospho Ser164) Antibody

• Background:

  This gene encodes a member of the tob/btg1 family of anti-proliferative proteins that have the potential to regulate cell growth. When exogenously expressed, this protein supresses cell growth in tissue culture. The protein undergoes phophorylation by a serine/threonine kinase, 90 kDa ribosomal S6 kinase. Interactions of this protein with the v-erb-b2 erythroblastic leukemia viral oncogene homolog 2 gene product p185 interferes with growth suppression. This protein inhibits T cell proliferation and transcription of cytokines and cyclins. The protein interacts with both mothers against decapentaplegic Drosophila homolog 2 and 4 to enhance their DNA binding activity. This interaction inhibits interleukin 2 transcription in T cells.

• NCBI Gene ID:

  10140

• Swiss Prot:

  P50616

• Accession Number:

  NP_001230806.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  TOB1 (phospho Ser164) antibody was raised against a peptide sequence around phosphorylation site of Serine 164 (A-V-S (p) -P-T) derived from Human TOB1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  40 kDa

• Fragment:

  IgG

• Specificity:

  The antibody detects endogenous levels of TOB1 only when phosphorylated at serine 164.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

• Symbol:

  TOB1

• NCBI Official Name:

  Transducer of ERBB2, 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Matsuda S., Oncogene 12:705-713 (1996) .

• Background Reference 02:

  Ebert L., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.

• Background Reference 03:

  Kalnine N., Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.

• Other Product Names:

  TOB, Transducer of erbB-2 1

• Tested Applications:

  WB, IHC

• Protein ID:

  345197216

• Physical Properties:

  Liquid