Pin1 (phospho Ser16) Antibody

• Background:

  Essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzes pSer/Thr-Pro cis/trans isomerizations. Down-regulates kinase activity of BTK. Can transactivate multiple oncogenes and induce centrosome amplification, chromosome instability and cell transformation. Required for the efficient dephosphorylation and recycling of RAF1 after mitogen activation. Binds and targets PML and BCL6 for degradation in a phosphorylation-dependent manner.

• NCBI Gene ID:

  5300

• Swiss Prot:

  Q13526

• Accession Number:

  NP_006212.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Pin1 (phospho Ser16) antibody was raised against a peptide sequence around phosphorylation site of serine16 (R-M-S (p) -R-S) derived from Human Pin1 .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  18 kDa

• Fragment:

  IgG

• Specificity:

  The antibody detects endogenous level of Pin1 only when phosphorylated at serine 16.

• Applications Notes:

  Western Blot: 1:500~1:1000

• Symbol:

  PIN1

• NCBI Official Name:

  Peptidylprolyl cis/trans isomerase, NIMA-interacting 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Lee YC, et al. (2013) J Cell Sci 126, 4862-72. Ando K, et al. (2013) Neurobiol Aging 34, 757-69. Nechama M, Lin CL, Richter JD (2013) Mol Cell Biol 33, 48-58.

• Other Product Names:

  PPIase Pin1, Rotamase Pin1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, EC 5.2.1.8

• Tested Applications:

  WB

• Protein ID:

  5453898

• Physical Properties:

  Liquid