MAP2K3 (phospho Thr222) Antibody

• Background:

  The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells.

• NCBI Gene ID:

  5606

• Swiss Prot:

  P46734

• Accession Number:

  NP_659731.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  MAP2K3 (phospho Thr222) antibody was raised against a peptide sequence around phosphorylation site of threonine 222 (A-K-T (p) -M-D) derived from Human MAP2K3 .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  39 kDa

• Fragment:

  IgG

• Specificity:

  The antibody detects endogenous levels of MAP2K3 only when phosphorylated at threonine 222.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

• Symbol:

  MAP2K3

• NCBI Official Name:

  Mitogen-activated protein kinase kinase 3

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Derijard B., Science 267:682-685 (1995) .

• Background Reference 02:

  Moriguchi T., J. Biol. Chem. 271:26981-26988 (1996) .

• Background Reference 03:

  The MGC Project Team; Genome Res. 14:2121-2127 (2004) .

• Other Product Names:

  MAP2K3, MAPKK 3, MEK3, MP2K3, PRKMK3

• Tested Applications:

  WB, IHC

• Protein ID:

  24638466

• Physical Properties:

  Liquid