Synaptotagmin (phospho Thr202) Antibody

• Background:

  The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca (2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse.

• NCBI Gene ID:

  6857

• Swiss Prot:

  P21579

• Accession Number:

  NP_001129277.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synaptotagmin (phospho Thr202) antibody was raised against a peptide sequence around phosphorylation site of threonine 202 (R-K-T (p) -L-N) derived from Human Synaptotagmin.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  60 kDa

• Fragment:

  IgG

• Specificity:

  The antibody detects endogenous levels of Synaptotagmin only when phosphorylated at threonine 202.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

• Symbol:

  SYT1

• NCBI Official Name:

  Synaptotagmin I

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  E Carafoli, Crit. Rev. Biochem. Mol. Biol., Apr 2001; 36: 107.

• Background Reference 02:

  Gry Houeland, J Neurophysiol, Jan 2007; 97: 134 - 143.

• Background Reference 03:

  Christelle Carteron, J. Cell Sci., Mar 2006; 119: 898 - 909.

• Background Reference 04:

  Vladimir I. Slepnev, Science, Aug 1998; 281: 821.

• Other Product Names:

  LAG, LAP18, STN1, Prosolin, stathmin

• Tested Applications:

  WB, IHC

• Protein ID:

  5032139

• Physical Properties:

  Liquid