PAK1 (phospho Thr212) Antibody

• Background:

  The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.

• NCBI Gene ID:

  5058

• Swiss Prot:

  Q13153

• Accession Number:

  NP_001122092.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  PAK1 (Phospho-Thr212) antibody was raised against a peptide sequence around phosphorylation site of threonine 212 (P-V-T (p) -P-T) derived from Human PAK1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific, Apoptosis, Cancer

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  68 kDa

• Specificity:

  This antibody detects endogenous level of PAK1 only when phosphorylated at threonine 212.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

• Symbol:

  PAK1

• NCBI Official Name:

  P21 protein (Cdc42/Rac) -activated kinase 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Alexander K, et al. (2004) Mol Cell Biol; 24: 2808-2819Thiel DA, et al. (2002) Curr Biol; 12:1227-1232Rashid T, et al. (2001) J. Biol. Chem; 276: 49043 - 49052.

• Other Product Names:

  PAKalpha, C1orf215, LAP18, OP18, p21-activated kinase 1, PAK-1, p65-PAK, Alpha-PAK

• Tested Applications:

  WB, IHC, IF

• Protein ID:

  190886457

• Physical Properties:

  Liquid