MAP2K4 Antibody

• Background:

  SAPK/Erk kinase (SEK1), also known as MKK4 or Jun kinase kinase (JNKK), activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflamatory cytokines (1-3) . Activation of SEK1 occurs through MEKK phosphorylation of serine and threonine residues at positions 257 and 261, respectively. Like MEK, SEK is a dual-specificity protein kinase that phosphorylates SAPK/JNK at a conserved T*PY* site in its activation loop (4) . Phosphorylation by Akt at Ser80 inhibits SEK1 and suppresses stress-activated signal transduction (5) .

• NCBI Gene ID:

  6416

• Swiss Prot:

  P45985

• Accession Number:

  NP_003001.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  MAP2K4 antibody was raised against a peptide sequence around aa.352~356 (E-S-K-R-P) derived from Human MAP2K4. .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  44 kDa

• Specificity:

  This antibody detects endogenous levels of total MAP2K4 protein.

• Applications Notes:

  Western Blot: 1:500~1:1000

• Symbol:

  MAP2K4

• NCBI Official Name:

  Mitogen-activated protein kinase kinase 4

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Davis, R. J. (1994) Trends Biochem. Sci. 19, 470-473. Sanchez, I. et al. (1994) Nature 372, 794-798. Yan, M. et al. (1994) Nature 372, 798-800. Kyriakis, J. M. et al. (1994) Nature 369, 156-160. Park, H. et al. (2002) J. Biol. Chem. 277, 2573-2578.

• Other Product Names:

  JNKK, MEK4, MKK4, SEK1, SKK1, JNKK1, SERK1, MAPKK4, PRKMK4, SAPKK1, SAPKK-1

• Tested Applications:

  WB

• Protein ID:

  4506889

• Physical Properties:

  Liquid