Shc1 (Ab 427) Antibody

• Background:

  Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span

• NCBI Gene ID:

  6464

• Swiss Prot:

  P29353

• Accession Number:

  NP_001123512.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Shc1 (Ab-427) antibody was raised against a peptide sequence around aa.425~429 (P-S-Y-V-N) derived from Human Shc1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  46, 52, 66 kDa

• Specificity:

  This antibody detects endogenous level of total Shc1 protein.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

• Symbol:

  SHC1

• NCBI Official Name:

  SHC (Src homology 2 domain containing) transforming protein 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Trampont P, et al. (2006) Mol Cell Biol; 26 (23) : 9035-9044. Patrussi L, et al. (2005) Oncogene; 24 (13) : 2218-2228van der Geer P, et al. (1996) Curr Biol ; 6 (11) : 1435-1444

• Other Product Names:

  SHC, SHCA, GLUH1, GLUR1, SH2 domain protein C1, SHC, SHC-transforming protein 1, Src homology 2 domain-containing-transforming protein C1

• Tested Applications:

  WB, IHC, IF

• Protein ID:

  194239664

• Physical Properties:

  Liquid