EIF4E (Ab 209) Antibody

• Background :

  Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.

• NCBI Gene ID :

  1977

• Swiss Prot :

  P06730

• Accession Number :

  NP_001124150.1

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  EIF4E (Ab-209) antibody was raised against a peptide sequence around aa. 207~211 (S-G-S-T-T) derived from Human eIF4E.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Other

• Purification :

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration :

  1 mg/mL

• Buffer :

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight :

  25 kDa

• Specificity :

  This antibody detects endogenous level of total elF4E protein.

• Applications Notes :

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

• Symbol :

  EIF4E

• NCBI Official Name :

  Eukaryotic translation initiation factor 4E

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Li BD, et al. (1998) Ann Surg; 227 (5) : 756-763Altmann M, et al. (1989) Nucleic Acids Res; 17 (18) : 7520De Gregorio E, et al. (2001) RNA; 7 (1) : 106-113Gu W, et al. (2004) Nucleic Acids Res; 32 (15) : 4448-4461Ohlmann T, et al. (1996) EMBO J; 15 (6) : 1371-1382

• Other Product Names :

  CBP, EIF4F, AUTS19, EIF4E1, EIF4EL1, A4, AD1, mRNA cap-binding protein, eIF-4F 25 kDa subunit

• Tested Applications :

  WB, IHC, IF

• Protein ID :

  194578907

• Physical Properties :

  Liquid