EIF4E (Ab 209) Antibody

CAT: 0223-79-374Size: 0.1 mgDry Ice: NoHazardous: No
CAT#:0223-79-374Size:0.1 mg
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Background
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
NCBI Gene ID
1977
Swiss Prot
P06730
Accession Number
NP_001124150.1
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
EIF4E (Ab-209) antibody was raised against a peptide sequence around aa. 207~211 (S-G-S-T-T) derived from Human eIF4E.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Other
Purification
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Concentration
1 mg/mL
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store antibody at -20˚ C for up to one year.
Calculated Molecular Weight
25 kDa
Specificity
This antibody detects endogenous level of total elF4E protein.
Applications Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Symbol
EIF4E
NCBI Official Name
Eukaryotic translation initiation factor 4E
NCBI Organism
Homo sapiens
Background Reference 01
Li BD, et al. (1998) Ann Surg; 227 (5) : 756-763Altmann M, et al. (1989) Nucleic Acids Res; 17 (18) : 7520De Gregorio E, et al. (2001) RNA; 7 (1) : 106-113Gu W, et al. (2004) Nucleic Acids Res; 32 (15) : 4448-4461Ohlmann T, et al. (1996) EMBO J; 15 (6) : 1371-1382
Other Product Names
CBP, EIF4F, AUTS19, EIF4E1, EIF4EL1, A4, AD1, mRNA cap-binding protein, eIF-4F 25 kDa subunit
Tested Applications
WB, IHC, IF
Protein ID
194578907
Physical Properties
Liquid

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