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EIF4G (Ab 1232) Antibody

CAT: 0223-79-422Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0223-79-422Size:0.1 mg
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Background
EIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with eIF3, mutually exclusive with EIF4A1 or EIFA2, EIF4E and through its N-terminus with PAPBC1. Interacts through its C-terminus with the serine/threonine kinases MKNK1, and with MKNK2. Appears to act as a scaffold protein, holding these enzymes in place to phosphorylate EIF4E. Non-phosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. EIF4G1/EIF4G3 interacts with PABPC1 to bring about circularization of the mRNA. Rapamycin can attenuate insulin stimulation mediated by FKBPs. Interacts with EIF4E3. Interacts with MIF4GD. Interacts with rotavirus A NSP3; in this interaction, NSP3 takes the place of PABPC1 thereby inducing shutoff of host protein synthesis
NCBI Gene ID
1981
Swiss Prot
Q04637
Accession Number
NP_004944.2
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
EIF4G (Ab-1232) antibody was raised against a peptide sequence around aa.1230~1234 (P-V-S-P-L) derived from Human eIF4G.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Other
Purification
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Concentration
1 mg/mL
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store antibody at -20˚ C for up to one year.
Calculated Molecular Weight
220 kDa
Specificity
This antibody detects endogenous level of total eIF4G protein.
Applications Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Symbol
EIF4G1
NCBI Official Name
Eukaryotic translation initiation factor 4 gamma, 1
NCBI Organism
Homo sapiens
Background Reference 01
De Gregorio, E. et al. (1998) RNA 4, 828-836. Ohlmann, T. et al. (1996) EMBO J. 15, 1371-1382. Borman, A. M. and Kean, K. M. (1997) Virology 237, 129-136. Gradi, A. et al. (1998) Mol Cell Biol 18, 334-42.
Other Product Names
P220, EIF4F, EIF4G, EIF4GI, PARK18, EIF-4G1, GAPD, CDABP0047, OK/SW-cl.12
Tested Applications
WB, IHC, IF
Protein ID
38201619
Physical Properties
Liquid

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