Mouse DHFR Antibody

• Background:

  Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.

• NCBI Gene ID:

  13361

• Swiss Prot:

  P00375

• Accession Number:

  P00375

• Host:

  Rabbit

• Reactivity:

  Mouse, Rat

• Immunogen:

  This Mouse DHFR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135-164 amino acids from the C-terminal region of mouse DHFR.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle, Obesity, Signal Transduction

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  22 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  Dhfr

• NCBI Official Name:

  Dihydrofolate reductase

• NCBI Organism:

  Mus musculus

• Background Reference 01:

  Cody, V., Proteins 65 (4), 959-969 (2006)

• Background Reference 02:

  Stone, D., J. Biol. Chem. 254 (2), 480-488 (1979)

• Other Product Names:

  Dihydrofolate reductase, Dhfr

• Tested Applications:

  WB, IHC-P

• Physical Properties:

  Liquid