Cdc6 Antibody

• Background:

  Human Cdc6 is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins.

• NCBI Gene ID:

  990

• Swiss Prot:

  Q99741

• Accession Number:

  Q99741

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This Cdc6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-61 amino acids from the N-terminal region of human Cdc6.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  63 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  CDC6

• NCBI Official Name:

  Cell division control protein 6 homolog

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Alexandrow, M.G., et al., Mol. Cell. Biol. 24 (4) :1614-1627 (2004) .

• Background Reference 02:

  Yim, H., et al., Mol. Biol. Cell 14 (10) :4250-4259 (2003) .

• Background Reference 03:

  Clay-Farrace, L., et al., EMBO J. 22 (3) :704-712 (2003) .

• Background Reference 04:

  Pelizon, C., et al., EMBO Rep. 3 (8) :780-784 (2002) .

• Other Product Names:

  Cell division control protein 6 homolog, CDC6-related protein, Cdc18-related protein, HsCdc18, p62 (cdc6), HsCDC6, CDC6, CDC18L

• Tested Applications:

  WB

• Protein ID:

  50400620

• Physical Properties:

  Liquid