CDC2 (phospho Thr161) Antibody

• Background:

  Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.

• NCBI Gene ID:

  983

• Swiss Prot:

  P06493

• Accession Number:

  NP_001163877.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  CDC2 (Phospho-Thr161) antibody was raised against a peptide sequence around phosphorylation site of threonine161 (T-Y-T (p) -H-E) derived from Human CDC2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific, Apoptosis, Cancer, Cell Cycle

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  34 kDa

• Specificity:

  This antibody detects endogenous level of CDC2 only when phosphorylated at threonine 161.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

• Symbol:

  CDK1

• NCBI Official Name:

  Cyclin-dependent kinase 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Ukomadu C, et al. (2003) J Biol Chem; 278 (7) : 4840-6. Morris MC, et al. (2002) J Biol Chem; 277 (26) : 23847-53. Brown NR, et al. (1999) J Biol Chem; 274 (13) : 8746-56. Liu Y, et al. (2004) J Biol Chem; 279 (6) : 4507-14.

• Other Product Names:

  CDC2, CDC28A, P34CDC2, C1orf215, LAP18, OP18, CDC28, CDC2A, CDK1, Cyclin-dependent kinase 1

• Tested Applications:

  WB, IHC

• Protein ID:

  281427278

• Physical Properties:

  Liquid