CDC2 (phospho Tyr15) Antibody

• Background:

  Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.

• NCBI Gene ID:

  983

• Swiss Prot:

  P06493

• Accession Number:

  NP_001163877.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  CDC2 (Phospho-Tyr15) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 15 (G-T-Y (p) -G-V) derived from Human CDC2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific, Apoptosis, Cancer, Cell Cycle

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  34 kDa

• Specificity:

  This antibody detects endogenous level of CDC2 only when phosphorylated at tyrosine 15.

• Applications Notes:

  Western Blot: 1:500~1:1000

• Symbol:

  CDK1

• NCBI Official Name:

  Cyclin-dependent kinase 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Y Gu, et al. (1992) EMBO J. 11 (11) : 3995–4005. P Jin, et al. (1996) Cell Biology, Vol 134: 963-970, Tyagi, et al. (2005) Alpna1Carcinogenesis, Volume 26: 1978-1987 (10) Xiang S. et al. (1997) The EMBO Journal 16: 182–192

• Other Product Names:

  CDC2, CDC28A, P34CDC2, CSBP, CSBP1, CSBP2, CSPB1, MXI2, SAPK2A, CDC28, CDC2A, CDK1, Cyclin-dependent kinase 1

• Tested Applications:

  WB

• Protein ID:

  281427278

• Physical Properties:

  Liquid