PARP1 Antibody

• Background:

  Involved in the base excision repair (BER) pathway, by catalyzing the poly (ADP-ribosyl) ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly (ADP- ribosyl) ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites.

• NCBI Gene ID:

  142

• Swiss Prot:

  P09874

• Accession Number:

  P09874

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 191-225 amino acids from human.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle, Obesity

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  113 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:500

• Symbol:

  PARP1

• NCBI Official Name:

  Poly [ADP-ribose] polymerase 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Uchida K., et al.Biochem. Biophys. Res. Commun. 148:617-622 (1987) .

• Background Reference 02:

  Kurosaki T., et al.J. Biol. Chem. 262:15990-15997 (1987) .

• Background Reference 03:

  Cherney B.W., et al.Proc. Natl. Acad. Sci. U.S.A. 84:8370-8374 (1987) .

• Background Reference 04:

  Auer B., et al.DNA 8:575-580 (1989) .

• Other Product Names:

  Poly [ADP-ribose] polymerase 1, PARP-1, 2.4.2.30, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD (+) ADP-ribosyltransferase 1, ADPRT 1, Poly[ADP-ribose] synthase 1, PARP1, ADPRT, PPOL

• Tested Applications:

  WB

• Protein ID:

  130781

• Physical Properties:

  Liquid